TGF-β3对体外培养的羊驼黑色素细胞的影响

doi:10.11843/j.issn.0366-6964.2015.05.009

Abstract

Abstract:

The aim of this study was to investigate the effect of TGF-β3 on the phenotype of alpaca melanocyte cultured in vitro.The melanocyte proliferation，gene expression of MITF，TYR and TYRP2 at the transcript and translation level were detected and melanin production was analyzed after the addition of TGF-β3 with different concentrations of 6.25，12.5，25，50 ng•mL^-1 in melanocyte medium.The results showed that：Compared to the normal melanocyte，the proliferation of melanocytes was inhibited within 30 h by the addition of 50 ng•mL^-1 TFG-β3 and then the amount of cells could be maintained without dependence on the concentration of TGF-β3 after 30 h； MITF， TYR and TYRP2 expression were down-regulated and melanin production also reduced after the addition of TGF-β3.The dose of 50 ng•mL^-1 TGF-β3 caused more significant effects.The results suggested that TGF-β3 had the important effects on the biology of alpaca melanocyte by regulating the expression of MITF，TYR，TYRP2 and melanin production.

CLC Number:

S852.2

LIU Yu，SHI Zhan-quan，JI Kai-yuan，YANG Shan-shan，FAN Rui-wen. The Effect of TGF-β3 on the Melanocyte Cultured in vitro of Alpaca[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 746-751.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.05.009

https://www.xmsyxb.com/EN/Y2015/V46/I5/746

References

［1］GRENSSNER A M，WEISKIRCHE N R.Modern pathogenetic concepts of liver fibrosis suggest stellate cells and TGF-β as major players and therapeutic targets［J］. J Cell Mol Med，2006，10：76-99.
［2］WAHL S M，WEN J，MOUTSOPOULOS N.TGF-beta：a mobile purveyor of immune privilege［J］.Immunol Rev，2006，213：213-227.
［3］LIM O，WANY Y，SANJABI S，et al.Transforming growth factor-beta regulation of immune responses［J］.Annu Rev Immunol，2006，24：99-146.
［4］曾东，余文林，杨传红，等.TGF-β各亚型与其受体在猪皮肤及毛囊中的表达［J］.中国美容医学，2009，18（5）：641-643.
ZENG D，YU W L，YANG C H，et al.Expression of TGF-β isoforms and their receptors in the pig skin and hair follicle［J］.Chinese Journal of Aesthetic Medicine，2009，18（5）：641-643.（in Chinese）
［5］FOITZIK K，PAUS R，DOETSCHMAN T，et al.The TGF-β2 isoform is both a required and sufficient 275 inducer of murine hair follicle morphogenesis［J］.Dev Biol，1999，212（2）：278-289.
［6］BARTRA M U，SPEER C P．The role of transforming growth factor beta in lung development and disease［J］.Chest，2004，125（2）：754-765.
［7］PARK S H．Fine tuning and cross-talking of TGF-beta singal by inhibitory smads［J］．Biochem Mol Biol，2005，38（1）：9-16.
［8］WILLERT K，NUSSE R.β-catenin：a key mediator of Wnt signaling［J］.Curr Opin Genet Devt，1998，8（1）：95-102.
［9］KUMASAKA M，GODING C R.β-catenin in the melanocyte lineage［J］.Pigment Cell Res，2003，16（3）：312-317.
［10］张晖，戈婷婷，彭沙沙，等.泰和乌骨鸡皮肤黑色素与多泡脂肪细胞的超微结构研究［J］.畜牧兽医学报，2014，45（5）：815-820.
ZHANG H，GE T T，PENG S S，et al.Ultrastructural studies Taihe Silky skin melanin and multivesicular fat cells［J］.Acta Veterinaria et Zootechnica Sinica，2014，45（5）：815-820.（in Chinese）
［11］HIBINO T，NISHIYAMA T.Role of TGF-beta2 in the human hair cycle［J］. J Dermatol Sci，2004，35（1）：9-18，285.
［12］BURTD W，LAWA S.Evolution of the transforming growth factor-beta superfamily［J］. Prog Growth Factor Res，1994，5（1）：99-107.
［13］LIMO-SANJABI S，FLAVELL R A.Transforming growth factor-βcontrols development，homeostasis，and tolerance of T cells by regulatory T cell-dependent and-independent mechanisms［J］.Immunity，2006，25（3）：455-471.
［14］SIMON J D，PELES D N.The red and the black［J］.Accounts Chem Res，2010，43（11）：1452-1460.
［15］AOKI H，MOTOHASHI T，YOSHIMURA N，et al.Cooperative and indispensable roles of Endothelin 3 and KIT signalings in melanocyte［J］.Dev Dynamics，2005，233：407-417.
［16］LIN J Y，FISHER D E.Melanocyte biology and skin pigmentation［J］.Nature，2007，445（22）：843-849.
［17］OTREBA M，ROK J，BUSZMAN E，et al.Regulation of melanogenesis：the role Of cAMP and MITF［J］.Postepy Hig Med Dosw，2012，66：33-40.
［18］AB-MALEK Z，SWOPE V B，SUZUKI I，et al.Mitogenic and melanogenic stimulation of normal human melanocytes by melanotopicpeptides［J］.Proc Natl Acad Sci USA，1995，92（51）：1789-1793.
［19］HEARING V J.Biochemical control of melanogenesis and melanosomal organization［J］. J Invest Dermatol，1999，4（1）：24-28.
［20］DEL MARMOL V，ITO S，JACKSON I，et al.TRP-1 expression correlates with eumelanogenesis in human pigment cells in culture［J］.Febs Letters，1993，327（3）：307-310.
［21］高莉，赵英虎，刘朝亮，等.酪氨酸酶相关蛋白1 调控黑色素形成的研究进展［J］.畜牧与饲料科学，2010，31（10）：114-116.
GAO L，ZHAO Y H，LIU C L，et al.Progress tyrosinase-related protein 1 regulated the formation of melanin［J］.Animal Husbandry and Feed Science，2010，31（10）：114-116.（in Chinese）
［22］VANCE K W，GODING C R.The transcription network regulating melanocyte development and melanoma［J］. Pigment Cell Res，2004，17（4）：318-325.

[1]	LIU Jie, CONG Wei, ZHAO Mindie, ZHAO Ruqian. Expression of GR and FKBP5 in Hippocampus and Hypothalamus of Arbor Acres and Rugao Yellow Broiler Chickens and Its Relationship with Stress Sensitivity [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4766-4776.
[2]	DAI Lingli, LIU Zaixia, GUO Lili, YANG Yanda, CHANG Chencheng, WANG Yu, SHI Caixia, WANG Yuzhen, ZHANG Wenguang. β-Hydroxybutyrate Mediated Epigenetic Modification and Its Molecular Mechanism of Regulating Inflammation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4095-4104.
[3]	ZHAO Huiying, YU Shiqiang, ZHAO Yuchao, JIANG Linshu. Mechanism of Liver-Adipose Tissue Crosstalk in the Development of Fatty Liver in Periparturient Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4105-4116.
[4]	CAO Xiyue, JI Xiaoxia, ZHANG Chonghao, ZHANG Yafeng, CHEN Yutao, ZHANG Yuanshu. Diminazene Aceturate Alleviates Pulmonary Fibrosis in Mice by Endogenous Activation of ACE2 to Inhibit AngⅡ-TGF-β1 Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2631-2640.
[5]	YANG Heng, LI Licai, FU Lin, XU Huihao, ZHANG Dezhi, LI Qianyong. Effect of Prostaglandin D₂ on Endocrine Function and Expression of Apoptosis-related Genes in Goat Luteal Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1652-1663.
[6]	JIN Meilin, LI Taotao, SUN Dongxiao, WEI Caihong. Research Progress of Epigenetic Regulation in Fat Deposition Mechanism of Livestock and Poultry [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 855-867.
[7]	YE Qianwen, CHEN Zhuo, LI Xin, SUN Yawei, JIN Xiaoye, LI Ziqian, WUMAIERJIANG·Yahefu, ZHONG Qi, MA Xuelian, YAO Gang. Dynamic Changes of Gut Microbiota Composition and Its Predicted Metabolism Function in One-month-old Sucking Lambs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1095-1108.
[8]	ZHANG Xiyu, ZHAI Pin, WANG Fan, DAI Yingying, ZHAO Bohao, CHEN Yang, WU Xinsheng. Expression and Function of KRT 16 in Hair Follicle Development of Angora Rabbit [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 157-167.
[9]	LIU Zhihao, JIA Peng, LAI Qi, DONG Lifeng, WU Qiujue, GAO Yanhua, TIAN Zhonghong, DIAO Qiyu. Characteristics of Rumen Fermentation and Methane Production in Different Parity Dry Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4296-4305.
[10]	CAO Xiyue, FU Yali, XIE Nana, ZHANG Yafeng, ZHANG Yuanshu. Inhibitory Effect and Mechanism of Endogenous Activation of Angiotensin Converting Enzyme 2 by Diminazene Aceturate on Cell Lipid Deposition [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2729-2738.
[11]	GUO Wenliang, XU Yuanqing, JIN Xiao, SHI Binlin. Moderating Role of Heat Shock Protein Under Inflammatory Response and Oxidative Stress Caused by Cold Stress [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1668-1677.
[12]	YUAN Jing, ZHANG Xinxin, ZHOU Di, YANG Shuai, ZHOU Jiayan, LIANG Aixin, YANG Liguo. Evaluation of the Effect of SS and CST Dual-expression DNA Vaccines with Attenuated Salmonella as Carrier in Immunizing Calves through Different Routes [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1914-1924.
[13]	SUN Yanjin, XUE Ya’nan, ZHONG Tao, WANG Linjie, LI Li, ZHANG Hongping, ZHAN Siyuan. The Function of HuR and Its Regulation on Muscle Growth and Development [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1345-1353.
[14]	DING Jun, FU Zilin, HE Junhao, DUAN Xiaowei, MA Lu, BU Dengpan. Research Progress of Milk-derived Exosomes [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1019-1029.
[15]	LI Yaqi, WANG Xianzhong, ZHANG Jiaojiao. Research Progress of Cell Proliferation and Apoptosis Regulated by Non-thermal Plasma and Its Application in Animal Husbandry [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 1-10.

The Effect of TGF-β3 on the Melanocyte Cultured in vitro of Alpaca

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments